IMMUNOMODULATORY POTENTIAL OF ETHANOL EXTRACT OF HENNA LEAF (Lawsonia Inermis L.) AGAINST MACROPHAGE PHAGOCYTOSIS IN MALE MICE (Mus Musculus)

EXTRACT OF HENNA LEAF
Wa Ode Gustiani Purnamasari 1 Indonesia , Fauziah Mulanna 2 Indonesia ROR , Wa Ode Yuliastri 2 Indonesia ROR , Asfani Yuhadi 2 Indonesia ROR
1 a:1:{s:5:"en_US";s:26:"UNIVERSITAS MANDALA WALUYA";}
2 Universitas Mandala Waluya ROR
Link Copied!
Published 2025-06-23
Vol. 7 No. 1 (2025) PAGES 30-36
DOI 10.36566/ijhsrd/Vol7.Iss1/293
Views / Downloads 606 497

Background: One of the herbal plants that can be used as an immunomodulator is henna leaves (Lawsonia inermis L.) that increase the function of the immune system. The purpose of this study was to determine the immunomodulatory potential of henna leaves on macrophage phagocytosis and to determine at what dose the ethanol extract of henna leaves has the potential for macrophage phagocytosis in male mice (Mus musculus).

Methods: The type of research used is analytical research using administering ethanol extract of henna leaves on increasing macrophage phagocytosis in male mice (Mus musculus) induced by Staphylococcus aureus, and to determine which dose is more effective in increasing phagocytosis using the phagocytosis test. The analysis method uses the ANOVA Statistical Test and the LSD Post Hoc Test.

Results: The study showed that the ethanol extract of henna leaves has an immunomodulatory effect based on the results of the One Way ANOVA statistical test and post hoc test (p-value <0.05). The dose of ethanol extract of henna leaves that has immunomodulatory potential is a dose of 100 mg/kgBW (34.33%), a dose of 200 mg/kgBW (46%), a dose of 300 mg/kgBW (74.66%) and a dose of 400 mg/kgBW (75.33%).

Conclusion: The study conclude that Ethanol extract of henna leaves has mmunomodulatory potential by increasing macrophage phagocytosis activity in male mice. Ethanol extract of henna leaves which is effective as an immunomodulator is 400 mg/kgBW which has a higher percentage of macrophage phagocytosis activity value compared to other dose variations, and is approximately the same as the positive control this is due to the number of phagocytic cells that actively carry out phagocytosis and is expressed as a percentage.

IMMUNOMODULATORY POTENTIAL OF ETHANOL EXTRACT OF HENNA LEAF (Lawsonia Inermis L.) AGAINST MACROPHAGE PHAGOCYTOSIS IN MALE MICE (Mus Musculus): EXTRACT OF HENNA LEAF . (2025). INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD), 7(1), 30-36. https://doi.org/10.36566/ijhsrd/Vol7.Iss1/293
Download data is not yet available.
  1. 1. Wahyuni), Muh.Hajrul Malaka), Adryan Fristiohady), Muhammad Ilyas Yusuf), and Sahidin. 2017. “Immunomodulatory Potential of Ethanol Extract of Torch Ginger Fruit (Etlingera Elatior (Jack) RMSmith) on Macrophage Phagocytosis Activity of Male Balb/C Strain Mice.” Pharmacon 6 (3): 350–55.
  2. 2. Amelia, Syahra, Wilda Amananti, and Rizki Febriyanti. 2021. “Comparison of Maceration and Reflux Methods on Antioxidant Activity of Soursop Leaf Extract (Annona Muricata L.).” Pharmacy of Harapan Bersama Polytechnic Tegal p-ISSN: 20 (e-ISSN: 2549-5062): 1–7.
  3. 3. Aripin, Ipin. 2019. “Value Education on the Immune System Concept Material.” Jurnal Bio Educatio 4 (1): 06–08. https://www.jurnal.unma.ac.id/index.php/BE/article/viewFile/1297/1207.
  4. 4. Darwin, Eryari, Dwitya Elvira, and Eka Fithra Elfi. 2021. Immunology and Infection. Andalas University Press. Vol. 5.
  5. 5. Devi, Silva, and Tuty Mulyani. 2017. “Antibacterial Activity Test of Ethanol Extract of Pacar Kuku Leaf (Lawsonia Inermis Linn) in Pseudomonas Aeruginosa” 1 (1): 30–35.
  6. 6. Edi, Imam Sarwo, Citra Esti Melodya, Sri Hidayati, and Endang Purwaningsih. 2021. “Differences in Inhibitory Power of Henna Leaf Extract (Lawsonia Inermis) Against the Growth of Porphyromonas Gingivalis Bacteria.” Journal of Dental Nursing Science (JIKG) 3 (2): 452–63. 2019. “Immunomodulatory Effects of Xestospongia Sp Sponge Ethanol Extract. Against Macrophage Phagocytosis Activity in Balb/C . Male Mice.” Jurnal Mandala Pharmacon Indonesia 5 (01): 15–30.
  7. 7. Zikriah. 2014. “Immunomodulatory Test of Black Cumin Ethanol Extract (Nigella Sativa L.) on Total Leukocyte Count, Lymphocyte Percentage, Monocyte Percentage and INTERLEUKIN-1β Levels in BALB/C Mice.” UIN Syarif Hidayatullah Jakarta.
  8. 8. Yuliastri, Wa Ode, Ajeng Diantini, Mohammad Ghozali, Idin Sahidin, and Muhammad Isrul. 2021. “Immunomodulatory Activity and Phytochemical Analysis of Hibiscus Sabdariffa L. Flower Fractions.” Journal of Applied Pharmaceutical Science 11 (11): 131–40.https://doi.org/10.7324/JAPS.2021.1101117.

Copyright (c) 2025 INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD)

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright Rights & Permissions Guide

Reproducing Published Material from other Publishers

It is absolutely essential that authors obtain permission to reproduce any published material (figures, schemes, tables or any extract of a text) which does not fall into the public domain, or for which they do not hold the copyright. Permission should be requested by the authors from the copyright holder (usually the Publisher, please refer to the imprint of the individual publications to identify the copyright holder).

Permission is required for:

  1. Your own works published by other Publishers and for which you did not retain copyright.
  2. Substantial extracts from anyones’ works or a series of works.
  3. Use of Tables, Graphs, Charts, Schemes and Artworks if they are unaltered or slightly modified.
  4. Photographs for which you do not hold copyright.

Permission is not required for:

  1. Reconstruction of your own table with data already published elsewhere. Please notice that in this case you must cite the source of the data in the form of either “Data from…” or “Adapted from…”.
  2. Reasonably short quotes are considered fair use and therefore do not require permission.
  3. Graphs, Charts, Schemes and Artworks that are completely redrawn by the authors and significantly changed beyond recognition do not require permission.

Obtaining Permission

In order to avoid unnecessary delays in the publication process, you should start obtaining permissions as early as possible. If in any doubt about the copyright, apply for permission. JNH cannot publish material from other publications without permission.

The copyright holder may give you instructions on the form of acknowledgement to be followed; otherwise follow the style: “Reproduced with permission from [author], [book/journal title]; published by [publisher], [year].’ at the end of the caption of the Table, Figure or Scheme.

Every mansucript should be accompanied by "Copyright Transfer Agreement" prior to the article publication

Dimension Badge

Downloads

Downloads
Crossmark Badge
Crossmark Badge
Section
Articles
Keywords
Immunomodulator ethanol extract henna leaves macrophage phagocytosis

Similar Articles

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)